Composition of platelet-rich plasma gel: A Western blot analysis

Objective: Platelet-rich plasma (PRP) gel is an autogenous blood-derived material that may be used as a regenerative agent of oral structures. The regenerative capacity of PRP is largely attributed to its composition, including many different growth factors. Thus far, no study has identified the molecular content of this gel. Therefore, it was the purpose of this study to assess the presence of different growth factors in PRP gel, using the Western blot technique.

Materials and methods: Blood samples were collected from 20 healthy donors and then processed to obtain PRP gel samples. The Western blot technique was used to determine the presence of the following growth factors: vascular endothelial growth factor (VEGF), platelet-derived growth factor (PDGF), transforming growth factor-beta (TGF-β1), fibroblast growth factor (FGF), insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF).

Results: Western blot analysis showed positive electrophoretic bands corresponding to molecular weights of the examined platelet growth factors. These bands were observed in every sample of PRP, demonstrating their presence as constituents of PRP gel.

Conclusion: Data from our study showed that PRP gel contains varying amounts of VEGF, TGF-β1, bFGF, PDGF-A, IGF-1 and EGF.